Sterile Compounding · Administration

Injection Guide

Video walkthroughs, step-by-step technique for pens and insulin syringes, and the needle size and route commonly used for each product in the catalog.

Video walkthroughs

How to fill and prime a peptide pen

Loading a cartridge, attaching the pen needle, priming out air, and dialing the dose.

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How to reconstitute a peptide vial

Adding bacteriostatic water down the vial wall, swirling (never shaking), and storing it.

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How to inject with an insulin syringe (SubQ)

Drawing the dose, clearing air bubbles, pinching the skin, and injecting subcutaneously.

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How to give an intramuscular (IM) injection

Deltoid and ventrogluteal landmarks, needle angle, and aspiration technique.

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Insulin syringe units explained

Reading U-100 markings so 'units' on the calculator match the marks on your syringe.

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Safe sharps handling and disposal

Never recapping, using a sharps container, and disposing of it properly.

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Each link opens a curated YouTube search so you always get current, working videos rather than a dead link. Prefer clips from pharmacists, nurses or clinics.

Filling a peptide pen

1Wash your hands and lay out the pen, a new pen needle, alcohol swabs and a sharps container.
2If the pen uses a cartridge, screw the cartridge holder onto the pen body until snug.
3Swab the rubber tip of the cartridge, then twist on a fresh pen needle and pull off both caps.
4Prime: dial 1–2 units, point the needle up, tap out bubbles and press the plunger until a bead of liquid appears at the tip. Repeat if nothing comes out.
5Dial your dose. Double-check the number in the window before you touch the pen to skin.
6Swab the site, pinch the skin, insert straight in at 90°, press the button fully and hold 10 seconds before withdrawing.
7Unscrew the needle and drop it straight into the sharps container — never recap or reuse it. Store the pen per its label (most mixed peptides go in the fridge).

Injecting with an insulin syringe

1Wash your hands. Swab both the peptide vial stopper and the bacteriostatic water stopper and let them dry.
2If the vial isn't mixed yet: draw your water with an 18–21G needle and run it slowly down the inside wall of the vial. Swirl gently — never shake.
3Pull the plunger of the insulin syringe to your dose in units, push that air into the vial, then invert the vial and draw your dose.
4Tap the barrel to float air bubbles to the tip and push them back into the vial. Re-check the unit mark against the calculator.
5Swab the injection site and let it dry. Pinch a fold of skin.
6Insert at 45–90° in one quick motion, inject slowly and steadily, then withdraw at the same angle.
7Press with a clean cotton pad — don't rub. Drop the whole syringe into a sharps container and rotate your site next time.

Needle sizes at a glance

Drawing / mixing: 18–21G × 1" — thick and fast for pulling water. Never inject with a draw needle.
Subcutaneous: 29–31G × 1/2" on a 0.5 mL U-100 insulin syringe (or a 4–6 mm pen needle).
Intramuscular: 23–25G × 1"–1.5" depending on the site and body composition.
Higher gauge = thinner needle. Use the thinnest needle that can move the fluid; thicker solutions need a lower gauge.

Needle size & instructions by product

GLP-1 & Metabolic

Product	Route	Recommended mix	Needle	Instructions
Retatrutide	Subcutaneous (SubQ)	2 mL water → 7.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.
Retatrutide	Subcutaneous (SubQ)	3 mL water → 10 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.
GLP-3 R	Subcutaneous (SubQ)	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.
Semaglutide	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.
Semaglutide	Subcutaneous (SubQ)	2 mL water → 7.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.
Semaglutide	Subcutaneous (SubQ)	2 mL water → 10 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.
Tirzepatide	Subcutaneous (SubQ)	3 mL water → 10 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.
Cagrilintide	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.
AOD-9604	Subcutaneous (SubQ)	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.
5-Amino-1MQ	Subcutaneous (SubQ)	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.
5-Amino-1MQ	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.
SLU-PP-322	Subcutaneous (SubQ)	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Weekly SubQ into abdomen, thigh or upper-outer buttock. Pinch, insert at 90°, inject slowly over 5–10 seconds, hold 5 seconds before withdrawing. Rotate sites weekly.

Healing & Tissue Repair

Product	Route	Recommended mix	Needle	Instructions
BPC-157	Subcutaneous — often near the affected area	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
BPC-157	Subcutaneous — often near the affected area	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
TB-500	Subcutaneous — often near the affected area	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
BPC-157 + TB-500	Subcutaneous — often near the affected area	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
KPV	Subcutaneous — often near the affected area	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
GLOW	Subcutaneous — often near the affected area	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
KLOW	Subcutaneous — often near the affected area	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
TB-4 Fragment	Subcutaneous — often near the affected area	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.

Growth Hormone & Secretagogues

Product	Route	Recommended mix	Needle	Instructions
CJC-1295 (with DAC)	Subcutaneous (SubQ)	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
CJC-1295 (without DAC)	Subcutaneous (SubQ)	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
CJC-1295 + Ipamorelin	Subcutaneous (SubQ)	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
Ipamorelin	Subcutaneous (SubQ)	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
Ipamorelin	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
Tesamorelin	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
Sermorelin	Subcutaneous (SubQ)	2 mL water → 4.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
Hexarelin	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
GHRP-2	Subcutaneous (SubQ)	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
GHRP-2	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
GHRP-2	Subcutaneous (SubQ)	2 mL water → 7.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
GHRP-6	Subcutaneous (SubQ)	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
HGH	Subcutaneous (SubQ)	1–2 mL water → 12–6 IU per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Reconstitute gently — aim the water down the vial wall and swirl, never shake. Inject SubQ into the abdomen, usually on an empty stomach before bed or fasted in the morning. Keep refrigerated after mixing.
HGH Fragment 176–191	Subcutaneous (SubQ)	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
Gonadorelin Acetate	Subcutaneous (SubQ)	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
Triptorelin	Subcutaneous (SubQ)	1 mL water → 2 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.

Longevity & Mitochondrial

Product	Route	Recommended mix	Needle	Instructions
Epithalon	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
Epithalon	Subcutaneous (SubQ)	5 mL water → 10 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
MOTS-c	Subcutaneous (SubQ)	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
NAD+	Subcutaneous (SubQ) — inject very slowly	5 mL water → 100 mg per mL (dilute well — NAD+ stings)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	NAD+ stings. Use extra water for a lower concentration, inject SubQ over 60+ seconds, and stop if flushing or chest tightness occurs. Many split the dose across two sites.
NAD+	Subcutaneous (SubQ) — inject very slowly	10 mL water → 100 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	NAD+ stings. Dilute well, inject SubQ over 60+ seconds and split large doses across sites or days.
FOXO4-DRI	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
SS-31 (Elamipretide)	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.

Immune Support

Product	Route	Recommended mix	Needle	Instructions
Thymosin Alpha-1	Subcutaneous (SubQ)	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
Thymosin Alpha-1	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
PNC-27	Subcutaneous (SubQ)	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
BL-34	Subcutaneous (SubQ)	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.

Cognitive / Nootropics

Product	Route	Recommended mix	Needle	Instructions
Selank	Subcutaneous or intranasal	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Commonly used intranasally or as a small SubQ abdominal injection with an insulin syringe.
Semax	Subcutaneous or intranasal	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Commonly used intranasally (a few drops per nostril) or as a small SubQ abdominal injection with an insulin syringe.
DSIP	Subcutaneous (SubQ)	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
Dihexa	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.

Melanotropins & Tanning

Product	Route	Recommended mix	Needle	Instructions
Melanotan I	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
Melanotan II	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
PT-141 (Bremelanotide)	Subcutaneous (SubQ)	2 mL water → 5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.

Skin, Hair & Cosmetic

Product	Route	Recommended mix	Needle	Instructions
GHK-Cu	Subcutaneous (or topical)	5 mL water → 10 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	SubQ into the abdomen, or applied topically. Copper peptides can sting and may leave a temporary blue tint at the site.
GHK-Cu	Subcutaneous (or topical)	10 mL water → 10 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	SubQ into the abdomen, or applied topically. Copper peptides can sting and may leave a temporary blue tint at the site.
Glutathione	Intramuscular (IM) or SubQ	3 mL water → 200 mg per mL	23–25G × 1"–1.5" for IM · 29–31G × 1/2" (0.5 mL U-100 insulin syringe) for SubQmix: 18–21G × 1" (draw only — never inject with it)	For IM, use the deltoid or ventrogluteal site: stretch the skin, insert at 90° in one smooth motion, inject slowly, withdraw and apply pressure. For SubQ, use the abdomen with an insulin syringe.
Glutathione	Intramuscular (IM) or SubQ	7.5 mL water → 200 mg per mL	23–25G × 1"–1.5" for IM · 29–31G × 1/2" (0.5 mL U-100 insulin syringe) for SubQmix: 18–21G × 1" (draw only — never inject with it)	For IM, use the deltoid or ventrogluteal site: stretch the skin, insert at 90° in one smooth motion, inject slowly, withdraw and apply pressure. For SubQ, use the abdomen with an insulin syringe.
Snap-8	Topical — not injected	Topical — mix per the supplier's directions; not injected	Not for injectionmix: —	Cosmetic topical peptide. Apply to skin; do not inject.
Lemon Bottle	Subcutaneous into the fat pad being treated	Ready to use — no reconstitution	30G × 1/2" (or a blunt cannula for larger areas)mix: 18–21G × 1" (draw only — never inject with it)	Multiple small SubQ deposits spread across the treatment area in a grid pattern, staying in the fat layer. Expect swelling for several days.

Sexual Health

Product	Route	Recommended mix	Needle	Instructions
Oxytocin Acetate	Subcutaneous (SubQ)	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.

Muscle & Performance

Product	Route	Recommended mix	Needle	Instructions
ACE-031	Subcutaneous — often near the affected area	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
IGF-1 LR3	Subcutaneous — often near the affected area	1 mL water → 100 mcg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
IGF-1 LR3	Subcutaneous — often near the affected area	1 mL water → 1 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
IGF-1 DES	Subcutaneous — often near the affected area	1 mL water → 1 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
MGF (Mechano Growth Factor)	Subcutaneous — often near the affected area	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
PEG-MGF	Subcutaneous — often near the affected area	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
Follistatin 344	Subcutaneous — often near the affected area	1 mL water → 1 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.
LL-37	Subcutaneous — often near the affected area	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Inject SubQ as close as is safely practical to the area of interest (never into a joint, tendon or vein). Pinch, 45–90°, inject slowly.

Hormones & Fertility

Product	Route	Recommended mix	Needle	Instructions
hCG	Subcutaneous (SubQ)	10 mL water → 1,000 IU per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Mix gently and refrigerate. Small SubQ abdominal injections, 2–3× weekly. Doses are in IU — the calculator switches to IU math for this vial.
Kisspeptin-10	Subcutaneous (SubQ)	2 mL water → 2.5 mg per mL	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.
VIP	Subcutaneous (SubQ)	Add 1–2 mL water (adjust to your target concentration)	29–31G × 1/2" (0.5 mL U-100 insulin syringe)mix: 18–21G × 1" (draw only — never inject with it)	Pinch a fold of fat on the abdomen (2 in. away from the navel), insert at 45–90°, inject slowly, withdraw and apply light pressure. Rotate sites each dose.

Vitamins & Injectable Compounds

Product	Route	Recommended mix	Needle	Instructions
Vitamin B12 (Methylcobalamin)	Intramuscular (IM) or SubQ	2 mL water → 5 mg per mL	23–25G × 1"–1.5" for IM · 29–31G × 1/2" (0.5 mL U-100 insulin syringe) for SubQmix: 18–21G × 1" (draw only — never inject with it)	For IM, use the deltoid or ventrogluteal site: stretch the skin, insert at 90° in one smooth motion, inject slowly, withdraw and apply pressure. For SubQ, use the abdomen with an insulin syringe.
Vitamin B12 (Cyanocobalamin)	Intramuscular (IM) or SubQ	2 mL water → 5 mg per mL	23–25G × 1"–1.5" for IM · 29–31G × 1/2" (0.5 mL U-100 insulin syringe) for SubQmix: 18–21G × 1" (draw only — never inject with it)	For IM, use the deltoid or ventrogluteal site: stretch the skin, insert at 90° in one smooth motion, inject slowly, withdraw and apply pressure. For SubQ, use the abdomen with an insulin syringe.
Vitamin B12 (Hydroxocobalamin)	Intramuscular (IM) or SubQ	2 mL water → 5 mg per mL	23–25G × 1"–1.5" for IM · 29–31G × 1/2" (0.5 mL U-100 insulin syringe) for SubQmix: 18–21G × 1" (draw only — never inject with it)	For IM, use the deltoid or ventrogluteal site: stretch the skin, insert at 90° in one smooth motion, inject slowly, withdraw and apply pressure. For SubQ, use the abdomen with an insulin syringe.
B-Complex (B1/B2/B3/B5/B6)	Intramuscular (IM)	Ready to use — no reconstitution	23–25G × 1"–1.5"mix: 18–21G × 1" (draw only — never inject with it)	Deep IM into the deltoid, ventrogluteal or vastus lateralis. Warm the vial in your hand, stretch the skin, insert at 90°, inject slowly (1–2 min for oily/thick solutions), then massage the site lightly.
Vitamin D3	Intramuscular (IM)	Ready to use (oil-based) — no reconstitution	23–25G × 1"–1.5"mix: 18–21G × 1" (draw only — never inject with it)	Deep IM into the deltoid, ventrogluteal or vastus lateralis. Warm the vial in your hand, stretch the skin, insert at 90°, inject slowly (1–2 min for oily/thick solutions), then massage the site lightly.
Biotin (B7)	Intramuscular (IM) or SubQ	2 mL water → 5 mg per mL	23–25G × 1"–1.5" for IM · 29–31G × 1/2" (0.5 mL U-100 insulin syringe) for SubQmix: 18–21G × 1" (draw only — never inject with it)	For IM, use the deltoid or ventrogluteal site: stretch the skin, insert at 90° in one smooth motion, inject slowly, withdraw and apply pressure. For SubQ, use the abdomen with an insulin syringe.
Vitamin C	Intramuscular (IM) or SubQ	10 mL water → 50 mg per mL	23–25G × 1"–1.5" for IM · 29–31G × 1/2" (0.5 mL U-100 insulin syringe) for SubQmix: 18–21G × 1" (draw only — never inject with it)	For IM, use the deltoid or ventrogluteal site: stretch the skin, insert at 90° in one smooth motion, inject slowly, withdraw and apply pressure. For SubQ, use the abdomen with an insulin syringe.
L-Carnitine	Intramuscular (IM)	10 mL water → 50 mg per mL	23–25G × 1"–1.5"mix: 18–21G × 1" (draw only — never inject with it)	Deep IM into the deltoid, ventrogluteal or vastus lateralis. Warm the vial in your hand, stretch the skin, insert at 90°, inject slowly (1–2 min for oily/thick solutions), then massage the site lightly.
MIC + B12 (Lipotropic)	Intramuscular (IM)	Ready to use — no reconstitution	23–25G × 1"–1.5"mix: 18–21G × 1" (draw only — never inject with it)	Deep IM into the deltoid, ventrogluteal or vastus lateralis. Warm the vial in your hand, stretch the skin, insert at 90°, inject slowly (1–2 min for oily/thick solutions), then massage the site lightly.
Taurine	Intramuscular (IM) or SubQ	10 mL water → 50 mg per mL	23–25G × 1"–1.5" for IM · 29–31G × 1/2" (0.5 mL U-100 insulin syringe) for SubQmix: 18–21G × 1" (draw only — never inject with it)	For IM, use the deltoid or ventrogluteal site: stretch the skin, insert at 90° in one smooth motion, inject slowly, withdraw and apply pressure. For SubQ, use the abdomen with an insulin syringe.
Magnesium	Intramuscular (IM)	10 mL water → 50 mg per mL	23–25G × 1"–1.5"mix: 18–21G × 1" (draw only — never inject with it)	Deep IM into the deltoid, ventrogluteal or vastus lateralis. Warm the vial in your hand, stretch the skin, insert at 90°, inject slowly (1–2 min for oily/thick solutions), then massage the site lightly.
CoQ10 (Ubiquinone)	Intramuscular (IM) or SubQ	5 mL water → 20 mg per mL	23–25G × 1"–1.5" for IM · 29–31G × 1/2" (0.5 mL U-100 insulin syringe) for SubQmix: 18–21G × 1" (draw only — never inject with it)	For IM, use the deltoid or ventrogluteal site: stretch the skin, insert at 90° in one smooth motion, inject slowly, withdraw and apply pressure. For SubQ, use the abdomen with an insulin syringe.
NMN	Intramuscular (IM) or SubQ	10 mL water → 50 mg per mL	23–25G × 1"–1.5" for IM · 29–31G × 1/2" (0.5 mL U-100 insulin syringe) for SubQmix: 18–21G × 1" (draw only — never inject with it)	For IM, use the deltoid or ventrogluteal site: stretch the skin, insert at 90° in one smooth motion, inject slowly, withdraw and apply pressure. For SubQ, use the abdomen with an insulin syringe.
Alpha-Lipoic Acid	Intramuscular (IM) or SubQ	5 mL water → 20 mg per mL	23–25G × 1"–1.5" for IM · 29–31G × 1/2" (0.5 mL U-100 insulin syringe) for SubQmix: 18–21G × 1" (draw only — never inject with it)	For IM, use the deltoid or ventrogluteal site: stretch the skin, insert at 90° in one smooth motion, inject slowly, withdraw and apply pressure. For SubQ, use the abdomen with an insulin syringe.

Injectable Support

Product	Route	Recommended mix	Needle	Instructions
Bacteriostatic Water	Diluent — not injected on its own	Diluent — draw what you need	—mix: 18–21G × 1" (draw only — never inject with it)	Swab the stopper, draw the volume you need with an 18–21G needle, then inject it slowly down the inside wall of the peptide vial.
Bacteriostatic Water	Diluent — not injected on its own	Diluent — draw what you need	—mix: 18–21G × 1" (draw only — never inject with it)	Swab the stopper, draw the volume you need with an 18–21G needle, then inject it slowly down the inside wall of the peptide vial.

Educational information only — not medical advice. Needle sizes and routes listed here are the ones commonly referenced for each compound; confirm anything you do with a qualified healthcare professional, and never reuse or share needles.